- Type
- Sources Sought
- Procedure
- Sources Sought
- Ref. number
- 78111dd8f1d54f3783a4e98804f52351
- NAICS
- 541714
Botulinum neurotoxins (BoNT/A�G) inhibit acetylcholine release at the neuromuscular junction through cleavage of SNARE proteins (e.g., SNAP-25, VAMP, syntaxin). Currently, BoNT characterization, including toxin potency a…
nd neutralization by medical countermeasures, is measured using mouse-based bioassays, primarily the Mouse Potency Assay (MPA) and Mouse Neutralization Assay (MNA), respectively. There is increasing scientific, ethical, and regulatory interest in transitioning to human-relevant in vitro systems that
can provide mechanistic insight while reducing reliance on animal testing. NMJ micro-physiological systems may provide a biologically relevant platform capable of measuring BoNT potency and evaluating countermeasure efficacy. BARDA is inte