LA2813C – University of Galway - RFT for the Supply of a FACS Machine for Choanoflagellates

must be microfluidics-based. • This system must have two lasers: 488nm and 561nm and at least 9 fluorescent channels. • The system must have the ability to do both two-way sorting as well as sorting into 96-well plates for isolation of single cells. • It must be possible to easily change the sheath

fluid used in sorting (in particular to seawater). • To preserve viability of choanoflagellate cells the system must operate at low pressure. • It is also essential to prevent cross-contamination between sorts so the entire fluid path shoul