The research group of Stefanos Stagkourakis will carry out a project focused on all-optical investigations that provide the capability of imaging and manipulating neuronal activity at the same time. Specifically, multiph…
oton imaging of Ca2+ and voltage indicators will be used to study neuron activity on large neuron populations in vivo and in vitro. Successful pursuit of this work requires simultaneous recording of activity and manipulation through multiphoton photostimulation of pre-selected neuron ensembles to st
udy a causal role of specific neuron subpopulations in behavior. A key component of the project is complementing the head-fixed in vivo neural activity and ensemble manipulation recordings with neuron activity imaging in freely moving anima