The Singe Cell Genomics Center (SCGC; https://scgc.bigelow.org) at the Bigelow Laboratory for Ocean Sciences is the only facility in the world capable of performing large-scale, integrated single-cell and single-viral-pa…
rticle genome and phenome analyses on diverse environmental microbiomes. The processing of exceedingly small DNA quantities makes single cell genomics highly susceptible to DNA contamination and amplification biases. SCGC has developed techniques to monitor and minimize methodological artifacts at e
very step of its workflow. Cell sorting and DNA amplification are performed in a cleanroom, and all consumables are decontaminated using in-house methods. Control wells on each microplate are used to detect potential DNA contamination. To p